UHRF1 | HUABIO HA723228 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

UHRF1

catalog :

HA723228

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-63

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of UHRF1 on different lysates with Rabbit anti-UHRF1 antibody (HA723228) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: F9 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 90 kDa Observed band size: 90-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HCT 116 cells labeling UHRF1 with Rabbit anti-UHRF1 antibody (HA723228) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UHRF1 antibody (HA723228) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of HL-60 cells labeling UHRF1 with Rabbit anti-UHRF1 antibody (HA723228) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UHRF1 antibody (HA723228) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723228

Target name :

UHRF1

Species reactivity :

Human,Mouse,Monkey

Applications :

WB,IF-Cell,FC,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human UHRF1 aa 1-350.

Uniprot id :

Q96T88>SwissProt: Q96T88 Human;SwissProt: Q8VDF2 Mouse;SwissProt: Q7TPK1 Rat

Host :

Rabbit

Clone number :

PSH10-63

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, HL-60 cell lysate, HepG2 cell lysate, Jurkat cell lysate, F9 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, HCT 116, HL-60.

Molecular wt :

Predicted band size: 90 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:10,000 ;FC: 1:1,000 ;IP: 1-2μg/sample ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723228_4.jpg

Pic legend4 :

Flow cytometric analysis of HCT 116 cells labeling UHRF1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723228, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723228_5.jpg

Pic legend5 :

UHRF1 was immunoprecipitated from 0.2 mg HL-60 cell lysate with HA723228 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723228 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HL-60 cell lysate (input) Lane 2: HA723228 IP in HL-60 cell lysate Lane 3: Rabbit IgG instead of HA723228 in HL-60 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 9 seconds; ECL: K1801

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723228_6.jpg

Pic legend6 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells with UHRF1 (HA723228) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage