Tau | HUABIO HA723209B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tau

catalog :

HA723209B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH10-42

reactivity :

human, mouse, rat

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723843, Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

product information

SKU :

HA723209B

Target name :

Tau

Species reactivity :

Human,Mouse,Rat

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Synthetic peptide within human Tau aa 57-68.

Uniprot id :

P10636-8>SwissProt: P10636-8 Human;SwissProt: P10637 Mouse

Host :

Rabbit

Clone number :

PSH10-42

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Subcellular location :

Cell membrane. Cytoplasm. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-41] to Human Phospho-Tau (T217) antibody (Capture) (HA723841) and Recombinant Human Phospho-Tau (T217) protein (HA211033) as the standard. The reference range value is 0.195-50 ng/mL. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-43] to Human Non-Phoshpho-Tau (T217) antibody (Capture) (HA723843) and Recombinant Human Phospho-Tau (T217) protein (HA210937) as the standard. The reference range value is 0.117-30 pg/mL. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-46] to Brain-derived tau/BD-tau antibody (Capture) (HA723847) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-10,000 pg/mL. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-48] to Tau antibody (Capture) (HA723850) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-5,000 pg/mL. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723209B_4.png

Pic legend4 :

Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723209B_5.png

Pic legend5 :

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723877) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

more info or order :

HUABIO product webpage