Western blot analysis of HOPX / HOD on different lysates with Rabbit anti-HOPX / HOD antibody (HA723199) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Rat lung tissue lysate Lane 6: Rat cerebellum tissue lysate Lane 7: Rat liver tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723199) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-HOPX / HOD antibody (HA723199) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723199) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.