FOXM1 | HUABIO HA723197 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FOXM1

catalog :

HA723197

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-30

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (HA723197) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: LO2 cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 110 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723197) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723197 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723197 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723197 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801

product information

SKU :

HA723197

Target name :

FOXM1

Species reactivity :

Human,Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human FOXM1 aa 1-400.

Uniprot id :

Q08050>SwissProt: Q08050 Human;SwissProt: O08696 Mouse

Host :

Rabbit

Clone number :

PSH10-30

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, LO2 cell lysate, Mouse testis tissue lysate.

Molecular wt :

Predicted band size: 84 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage