product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-RICTOR (T1135)
catalog :
HA723161
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH09-92
reactivity :
human, mouse
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA723161 image 1
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA723161 image 2
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: C2C12 starved overnight cell lysate Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA723161
Target name :
Phospho-RICTOR (T1135)
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Thr1135 of Human RICTOR.
Uniprot id :
Q6R327>SwissProt: Q6R327 Human;SwissProt: Q6QI06 Mouse
Host :
Rabbit
Clone number :
PSH09-92
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate.
Molecular wt :
Predicted band size: 192 kDa
Subcellular location :
Cytosol, Golgi apparatus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!