Human TREM2 | HUABIO HA723149 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human TREM2

catalog :

HA723149

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-83

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human TREM2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723149) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM2 protein (HA210647) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723151, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native TREM2 in human cell culture supernatant samples. Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations and interpolated from the TREM2 standard curves. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 4,912 pg/mL in THP-1 cell culture supernatant, undetectable in Jurkat cell culture supernatant.

image 3 :

Interpolated concentrations of spiked TREM2 in cell culture media samples. The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

product information

SKU :

HA723149

Target name :

Human TREM2

Species reactivity :

Human

Applications :

ELISA(Cap),ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TREM2 Protein aa 19-174 (HA210647).

Uniprot id :

Q9NZC2>SwissProt: Q9NZC2 Human

Host :

Rabbit

Clone number :

PSH09-83

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human TREM2 Protein (HA210647).

Subcellular location :

Secreted, Cell membrane.

Concentration :

1 mg/ml

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-85] to Human TREM2 antibody (Detector) (HA723151) and Recombinant Human TREM2 protein (HA210647) as the standard. The reference range value is 39-5,000pg/ml. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-84] to Human TREM2 antibody (Capture) (HA723150) and Recombinant Human TREM2 protein (HA210647) as the standard. The reference range value is 39-5,000pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723149_4.png

Pic legend4 :

Sandwich ELISA analysis of Human TREM2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723150) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM2 protein (HA210647) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723149, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723149_5.png

Pic legend5 :

Interpolated concentrations of native TREM2 in human cell culture supernatant samples. Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations and interpolated from the TREM2 standard curves. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 4,597 pg/mL in THP-1 cell culture supernatant, undetectable in Jurkat cell culture supernatant.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723149_6.png

Pic legend6 :

more info or order :

HUABIO product webpage