Phospho-AKT2 (S474) | HUABIO HA723108 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-AKT2 (S474)

catalog :

HA723108

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-46

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-AKT2 (S474) on different lysates with Rabbit anti-Phospho-AKT2 (S474) antibody (HA723108) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate Lane 3: HEK-293 cell lysate Lane 4: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate Lane 7: C6 cell lysate Lane 8: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: Lane 1-6: 3 minutes; Lane 7-8: 12 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723108) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of MCF7 cells (left) / MCF7 cells treated with 50ng/mL Calyculin A for 45 minutes (right) labeling Phospho-AKT2 (S474). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723108, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of C6 cells (left) / C6 cells treated with 100ng/mL Calyculin A for 1 hours (right) labeling Phospho-AKT2 (S474). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723108, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723108

Target name :

Phospho-AKT2 (S474)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser474 of human AKT2.

Uniprot id :

P31751>SwissProt: P31751 Human;SwissProt: Q60823 Mouse;SwissProt: P47197 Rat

Host :

Rabbit

Clone number :

PSH09-46

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, HEK-293 cell lysate, HEK-293 treated with 50μM LY294002 for 6 hours cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate, C6 cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, MCF7 cells treated with 50ng/mL Calyculin A for 45 minutes, C6 cells treated with 100ng/mL Calyculin A for 1 hours.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane, Early endosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage