Phospho-Tau (T217) | HUABIO HA723091 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Tau (T217)

catalog :

HA723091

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-33

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/2,000 dilution. Lane 1: 293T transfected with Tau cell lysate Lane 2: 293T transfected with Tau (mutated T217A) cell lysate (negative) Lysates/proteins at 15 µg/Lane. Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723091) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of 293T cells labeling Phospho-Tau (T217) with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/500 dilution. 293T cells, transfected with empty control (top, negative) / Tau (middle, positive) / Tau (mutated T217A) (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723091) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA723091

Target name :

Phospho-Tau (T217)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr217 of human Tau-F (P10636-8).

Uniprot id :

P10636-8>SwissProt: P10636-8 Human;SwissProt: P10637 Mouse

Host :

Rabbit

Clone number :

PSH09-33

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysate, Rat brain tissue lysate, mouse brain tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:500 ;IHC-P: 1:50 ;IHC-Fr: 1:50

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723091_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue untreated / treated with λpp with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723091) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723091_5.jpg

Pic legend5 :

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723091, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723091_6.jpg

Pic legend6 :

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/1,000 dilution. Lane 1: WT mouse cortex tissue#4 Lane 2: WT mouse cortex tissue#6 Lane 3: WT mouse cortex tissue#24 Lane 4: WT mouse cortex tissue#27 Lane 5: Tau P301S（PS19）mouse cortex tissue#1 Lane 6: Tau P301S（PS19）mouse cortex tissue#15 Lane 7: Tau P301S（PS19）mouse cortex tissue#19 Lane 8: Tau P301S（PS19）mouse cortex tissue#22 Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723091) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage