Human E-Cadherin | HUABIO HA723056 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human E-Cadherin

catalog :

HA723056

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0287

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human E-Cadherin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723055) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human E-Cadherin protein (HA210886) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723056, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native E-Cadherin in Jurkat and MCF-7 cell culture supernatant. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 50% and Jurkat cell culture supernatnat 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was undetectable in Jurkat cell culture supernatant and 13.7 ng/ml in MCF-7 cell culture supernatant.

image 3 :

Interpolated concentrations of native E-Cadherin in human urine and human serum samples. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are human urine 13% and human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 33.1 ng/ml in human urine and 137.0 ng/ml in human serum.

product information

SKU :

HA723056

Target name :

Human E-Cadherin

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human E-cadherin aa 155-707.

Uniprot id :

P12830>SwissProt: P12830 Human

Host :

Rabbit

Clone number :

SY0287

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human E-Cadherin protein (HA210886).

Subcellular location :

Cell junction. Cell membrane. Cytoplasm. Endosome. Golgi apparatus. Membrane.

Concentration :

1 mg/ml.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-02] to Human E-Cadherin antibody (Capture) (HA723055) and Recombinant Human E-Cadherin protein (HA210886) as the standard. The reference range value is 156-20,000pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723056_4.png

Pic legend4 :

Interpolated concentrations of spiked E-Cadherin in human cell culture media samples. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage