product summary
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company name :
HUABIO
product type :
antibody
product name :
Cystatin F
catalog :
HA723053
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH09-00
reactivity :
human, mouse
application :
western blot, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA723053) at 1/2,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 20 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 3 :
Immunofluorescence analysis of frozen mouse brain tissue (negative) with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
product information
SKU :
HA723053
Target name :
Cystatin F
Species reactivity :
Human,Mouse
Applications :
WB,IHC-Fr
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Cystatin F aa 1-145.
Uniprot id :
O76096>SwissProt: O76096 Human;SwissProt: O89098 Mouse
Host :
Rabbit
Clone number :
PSH09-00
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
U-937 cell lysate, mouse spleen tissue.
Molecular wt :
Predicted band size: 16 kDa
Subcellular location :
Secreted, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-Fr: 1:100
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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