product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-MYPT1 (T696)
catalog :
HA723049
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH08-96
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Phospho-MYPT1 (T696) on different lysates with Rabbit anti-Phospho-MYPT1 (T696) antibody (HA723049) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM Y-27632 for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 115 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723049) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA723049
Target name :
Phospho-MYPT1 (T696)
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Thr696 of Human MYPT1.
Uniprot id :
O14974>SwissProt: O14974 Human;SwissProt: Q9DBR7 Mouse;SwissProt: Q10728 Rat
Host :
Rabbit
Clone number :
PSH08-96
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate.
Molecular wt :
Predicted band size: 115 kDa
Subcellular location :
Cytoplasm, cytoskeleton, stress fiber.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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