Ghrelin | HUABIO HA723029 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ghrelin

catalog :

HA723029

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-77

reactivity :

human, mouse

application :

immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse stomach tissue with Rabbit anti-Ghrelin antibody (HA723029) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723029, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Immunofluorescence analysis of frozen mouse liver tissue (negative) with Rabbit anti-Ghrelin antibody (HA723029) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723029, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Ghrelin antibody (HA723029) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723029) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723029

Target name :

Ghrelin

Species reactivity :

Human,Mouse

Applications :

IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Ghrelin aa 1-117.

Uniprot id :

Q9UBU3>SwissProt: Q9UBU3 Human;SwissProt: Q9EQX0 Mouse

Host :

Rabbit

Clone number :

PSH08-77

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human stomach tissue, mouse stomach tissue.

Molecular wt :

Predicted band size: 13 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:25,000-1:50,000 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723029_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Ghrelin antibody (HA723029) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723029) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723029_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ghrelin antibody (HA723029) at 1/25,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723029) at 1/25,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage