MAP2 | HUABIO HA723025 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAP2

catalog :

HA723025

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-73

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA723025, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA723025, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required

product information

SKU :

HA723025

Target name :

MAP2

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey

Applications :

WB,IHC-P,IHC-Fr,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAP2 aa 1-600.

Uniprot id :

P11137>SwissProt: P11137 Human;SwissProt: P20357 Mouse;SwissProt: P15146 Rat

Host :

Rabbit

Clone number :

PSH08-73

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse primary neural cells, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 200 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, dendrite.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:500-1:8,000 ;IHC-Fr: 1:500 ;IF-Cell: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723025_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723025_5.jpg

Pic legend5 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723025_6.jpg

Pic legend6 :

Immunocytochemistry analysis of mouse primary neural cells labeling MAP2 with Rabbit anti-MAP2 antibody (HA723025) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAP2 antibody (HA723025) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723025_7.jpg

Pic legend7 :

Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA723025) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse kidney tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 200 kDa Observed band size: 150-300 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723025_8.jpg

Pic legend8 :

Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA723025) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 200 kDa Observed band size: 150-300 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA723025_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723025) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA723025_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723025) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA723025_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723025) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage