product summary
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company name :
HUABIO
product type :
antibody
product name :
HMGCS1
catalog :
HA722997
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH08-51
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA722997 image 1
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA722997) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: HT-29 cell lysate Lane 4: A549 cell lysate Lane 5: COS-1 cell lysate Lane 6: C2C12 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722997) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA722997 image 2
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA722997 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722997 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: HA722997 IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of HA722997 in HT-29 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 37 seconds; ECL: K1801
product information
SKU :
HA722997
Target name :
HMGCS1
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human HMGCS1 aa 421-520.
Uniprot id :
Q01581>SwissProt: Q01581 Human;SwissProt: Q8JZK9 Mouse;SwissProt: P17425 Rat
Host :
Rabbit
Clone number :
PSH08-51
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Rat liver tissue lysate.
Molecular wt :
Predicted band size: 57 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!