product summary
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company name :
HUABIO
product type :
antibody
product name :
Rubicon / Baron
catalog :
HA722969
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH08-25
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :

Western blot analysis of Rubicon / Baron on different lysates with Rabbit anti-Rubicon / Baron antibody (HA722969) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: A549 cell lysate
Lane 3: NCI-H1299 cell lysate
Lane 4: HeLa cell lysate
Lane 5: 293T cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: C6 cell lysate
Lane 10: Mouse spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 109 kDa
Observed band size: 109 kDa
Exposure time: Lane 1: 18 seconds; Lane 2-10: 1 minute 16 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722969) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA722969
Target name :
Rubicon / Baron
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Rubicon aa 1-350.
Uniprot id :
Q92622>SwissProt: Q92622 Human;SwissProt: Q80U62 Mouse;Entrez Gene: 303885 Rat
Host :
Rabbit
Clone number :
PSH08-25
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Raji cell lysate, A549 cell lysate, NCI-H1299 cell lysate, HeLa cell lysate, 293T cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate.
Molecular wt :
Predicted band size: 109 kDa
Subcellular location :
Late endosome, Lysosome, Early endosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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