IRF9 | HUABIO HA722963 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IRF9

catalog :

HA722963

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-20

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IRF9 on different lysates with Rabbit anti-IRF9 antibody (HA722963) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate Lane 3: Daudi cell lysate Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM IFN-α1 for 24 hours cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 48 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IRF9 on different lysates with Rabbit anti-IRF9 antibody (HA722963) at 1/1,000 dilution. Lane 1: Human IRF9 recombinant protein, 30ng/Lane Lane 2: Human IRF8 recombinant protein, 30ng/Lane Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722963) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells treated with 10ng/mL IFN-α1 for 16 hours labeling IRF9 with Rabbit anti-IRF9 antibody (HA722963) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRF9 antibody (HA722963) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA722963

Target name :

IRF9

Species reactivity :

Human

Applications :

WB,IF-Cell,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IRF9 aa 1-393.

Uniprot id :

Q00978>SwissProt: Q00978 Human

Host :

Rabbit

Clone number :

PSH08-20

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Jurkat cell lysate, Jurkat treated with 100nM IFN-α1 for 24 hours cell lysate, THP-1 cell lysate, HeLa cells treated with 10ng/mL IFN-α1 for 16 hours.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample ;ChIP: Use 2~5 μg for 25 μg of chromatin.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722963_4.jpg

Pic legend4 :

IRF9 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722963 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722963 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722963 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722963 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1802

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722963_5.jpg

Pic legend5 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 10ng/mL IFN-α1 for 16 hours with IRF9 (HA722963) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage