Engrailed 1 | HUABIO HA722960 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Engrailed 1

catalog :

HA722960

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-17

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722960) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722960) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of SH-SY5Y cells labeling Engrailed 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722960, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722960

Target name :

Engrailed 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Engrailed 1 aa 1-392.

Uniprot id :

Q05925>SwissProt: Q05925 Human;SwissProt: P09065 Mouse;Entrez Gene: 685360 Rat

Host :

Rabbit

Clone number :

PSH08-17

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage