Phospho-AKT (T308) | HUABIO HA722951 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-AKT (T308)

catalog :

HA722951

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-10

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-AKT (T308) on different lysates with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: 293T cell lysate Lane 6: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722951) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308) with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722951, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722951

Target name :

Phospho-AKT (T308)

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr308 of Human AKT1.

Uniprot id :

P31749>SwissProt: P31749 Human

Host :

Rabbit

Clone number :

PSH08-10

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, 293T treated with 100nM Calyculin A for 15 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage