Human NCAM1 | HUABIO HA722947 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human NCAM1

catalog :

HA722947

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-08

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human NCAM1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722946) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human NCAM1 protein (HA210820) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722947, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native NCAM1 in human samples. Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 320 ng/mL in human serum.

image 3 :

Interpolated concentrations of native NCAM1 in human cell culture supernatant samples. Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 4621 pg/mL in SH-SY5Y cell culture supernatant samples.

product information

SKU :

HA722947

Target name :

Human NCAM1

Species reactivity :

Human

Applications :

ELISA(Det),ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NCAM1 protein aa 20-718 (HA210820).

Uniprot id :

P13591>SwissProt: P13591 Human

Host :

Rabbit

Clone number :

PSH08-08

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human NCAM1 protein (HA210820).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-07] to Human NCAM1 antibody (Capture) (HA722946) and recombinant Human NCAM1 protein as the standard (HA210820). The reference range value is 74-6,000 pg/ml. ;ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-09] to Human NCAM1 antibody (Detector) (HA722949) and recombinant Human NCAM1 protein as the standard (HA210820). The reference range value is 74-6,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722947_4.png

Pic legend4 :

Interpolated concentrations of spiked NCAM1 in cell culture media samples. The concentrations of NCAM1 were measured in duplicates, interpolated from the NCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722947_5.png

Pic legend5 :

Sandwich ELISA analysis of Human NCAM1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722947) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human NCAM1 protein (HA210820) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722949, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722947_6.png

Pic legend6 :

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722947_7.png

Pic legend7 :

Interpolated concentrations of native NCAM1 in human cell culture supernatant samples. Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 3503 pg/mL in SH-SY5Y cell culture supernatant samples.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722947_8.png

Pic legend8 :

more info or order :

HUABIO product webpage