Human TRAIL | HUABIO HA722943 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human TRAIL

catalog :

HA722943

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-05

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human TRAIL matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722943) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAIL protein as the standard (HA210893) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722944, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native TRAIL in 786-0 and Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 11,821 pg/mL in 786-0 cell extract, There was no detectable signal in Hela cell extract.

image 3 :

Interpolated concentrations of native TRAIL in cell culture supernatant samples. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 260 pg/mL in 786-0 cell supernanant. There was no detectable signal in Hela cell supernatant.

product information

SKU :

HA722943

Target name :

Human TRAIL

Species reactivity :

Human

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TRAIL aa 114-281.

Uniprot id :

P50591>SwissProt: P50591 Human

Host :

Rabbit

Clone number :

PSH08-05

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human TRAIL protein as the standard (HA210893).

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-06] to Human TRAIL antibody (Detector) (HA722944) and recombinant Human TRAIL protein as the standard (HA210893). The reference range value is 16.5-4,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722943_4.png

Pic legend4 :

Interpolated concentrations of spiked TRAIL in cell culture media samples. The concentrations of TRAIL were measured in duplicates, interpolated from the TRAIL standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage