Phospho-Nucleolin (T76) | HUABIO HA722942 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Nucleolin (T76)

catalog :

HA722942

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE64-03

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Nucleolin (T76) on different lysates with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate (20 µg/Lane) Lane 3: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Predicted band size: 77 kDa Observed band size: 105 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722942) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722942) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722942) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722942

Target name :

Phospho-Nucleolin (T76)

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr76 of Human Nucleolin.

Uniprot id :

P19338>SwissProt: P19338 Human

Host :

Rabbit

Clone number :

JE64-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, human tonsil tissue, human kidney cancer tissue.

Molecular wt :

Predicted band size: 77 kDa

Subcellular location :

Nucleus, nucleolus;Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage