TBR2 | HUABIO HA722918 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TBR2

catalog :

HA722918

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-88

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA722918) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722918) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 73/85 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722918

Target name :

TBR2

Species reactivity :

Human,Mouse,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TBR2 aa 1-300.

Uniprot id :

O95936>SwissProt: O95936 Human;SwissProt: O54839 Mouse

Host :

Rabbit

Clone number :

PSH07-88

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NK-92 cell lysate, Mouse embryo tissue lysate, E14.5 mouse embryonic brain tissue.

Molecular wt :

Predicted band size: 73 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722918_4.jpg

Pic legend4 :

Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 73/85 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage