Nephrin | HUABIO HA722886 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Nephrin

catalog :

HA722886

quantity :

100μl

price :

385.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-70

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722886, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Western blot analysis of Nephrin on different lysates with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 135 kDa Observed band size: 200 kDa Exposure time: 1 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722886) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722886

Target name :

Nephrin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Nephrin aa 1-1,050.

Uniprot id :

O60500>SwissProt: O60500 Human;SwissProt: Q9QZS7 Mouse;SwissProt: Q9R044 Rat

Host :

Rabbit

Clone number :

PSH07-70

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit multiclonal Antibody

Positive control :

Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 135 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:3,000 ;IF-Tissue: 1:200-1:1,000 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE),Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722886_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722886_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722886_6.jpg

Pic legend6 :

Western blot analysis of Nephrin on different lysates with Rabbit anti-Nephrin antibody (HA722886) at 1/5,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse kidney tissue lysate treated with deglycosylation Lysates/proteins at 14.4 µg/Lane. Predicted band size: 135 kDa Observed band size: 200/135 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722886) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage