Human IL-13R alpha 2 | HUABIO HA722881 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human IL-13R alpha 2

catalog :

HA722881

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-64

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722881, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract.

image 3 :

Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract.

product information

SKU :

HA722881

Target name :

Human IL-13R alpha 2

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human IL-13R alpha 2 aa 27-343.

Uniprot id :

Q14627>SwissProt: Q14627 Human

Host :

Rabbit

Clone number :

PSH07-64

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rrecombinant Human IL-13R alpha 2 protein (HA210969).

Subcellular location :

Extracellular

Concentration :

1 mg/mL

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-63] to Human IL-13R alpha 2 antibody (Capture) (HA722880) and recombinant Human IL-13R alpha 2 protein (HA210969) as the standard. The reference range value is 0.247-20 ng/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722881_4.png

Pic legend4 :

Interpolated concentrations of spiked IL-13 R alpha 2 in human cell culture media samples. The concentrations of IL-13 R alpha 2 were interpolated from the IL-13 R alpha 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.

more info or order :

HUABIO product webpage