product summary
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company name :
HUABIO
product type :
antibody
product name :
Connexin 32 / GJB1
catalog :
HA722869
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE54-70
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 26 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/2,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse spleen tissue lysate (negative)
Lane 3: Rat liver tissue lysate
Lane 4: Rat spleen tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 48 kDa
Exposure time: 1 minute 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA722869
Target name :
Connexin 32 / GJB1
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Connexin 32 / GJB1 aa 46-75 / 283.
Uniprot id :
P08034>SwissProt: P08034 Human;SwissProt: P28230 Mouse;SwissProt: P08033 Rat
Host :
Rabbit
Clone number :
JE54-70
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MCF7 cell lysate, 293T cell lysate, Human lung tissue lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Molecular wt :
Predicted band size: 32 kDa
Subcellular location :
Cell membrane, Cell junction, gap junction.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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