product summary
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company name :
HUABIO
product type :
antibody
product name :
LAMP1
catalog :
HA722827
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH07-39
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA722827 image 1
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: HeLa cell lysate Lane 7: HeLa cell lysate treated with deglycosylation Lane 8: NIH/3T3 cell lysate Lane 9: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 120/44 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA722827 image 2
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: L6 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
HUABIO HA722827 image 3
Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
product information
SKU :
HA722827
Target name :
LAMP1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,IHC-Fr,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within mouse LAMP1 aa 371-406.
Uniprot id :
P11279>SwissProt: P11279 Human;SwissProt: P11438 Mouse;SwissProt: P14562 Rat
Host :
Rabbit
Clone number :
PSH07-39
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human kidney tissue, mouse colon tissue, mouse kidney tissue, mouse testis tissue, rat kidney tissue.
Molecular wt :
Predicted band size: 44 kDa
Subcellular location :
Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000 ;IHC-P: 1:10,000 ;IF-Tissue: 1:500-1:2,000 ;IHC-Fr: 1:1,000 ;IP: 1-2μg/sample
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722827_4.jpg
Pic legend4 :
Application: IHC-Fr Species: Rat Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722827_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA722827_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA722827_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA722827_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA722827_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA722827_10.jpg
Pic legend10 :
LAMP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722827 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722827 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722827 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722827 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!