LAMC2 | HUABIO HA722815 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LAMC2

catalog :

HA722815

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-27

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA722815) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (low expression) (20 µg/Lane) Lane 3: HepG2 cell lysate (low expression) (20 µg/Lane) Lane 4: HaCaT cell lysate (low expression) (20 µg/Lane) Lane 5: NCI-H441 cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Mouse stomach tissue lysate (40 µg/Lane) Lane 8: Rat skin tissue lysate (40 µg/Lane) Lane 9: Rat stomach tissue lysate (40 µg/Lane) Predicted band size: 131 kDa Observed band size: 100/140 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722815) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA722815) at 1/5,000 dilution. Lane 1: KYSE-150-parental cell lysate Lane 2: KYSE-150-LAMC2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 140/90 kDa Observed band size: 140/90 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722815)) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LAMC2 antibody (HA722815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722815

Target name :

LAMC2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human LAMC2 aa 550-1,193.

Uniprot id :

Q13753>SwissProt: Q13753 Human;SwissProt: Q61092 Mouse;Entrez Gene: 192362 Rat

Host :

Rabbit

Clone number :

PSH07-27

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, A549 cell lysate, HaCaT cell lysate, NCI-H441 cell lysate, Mouse skin tissue lysate, Mouse stomach tissue lysate, Rat skin tissue lysate, Rat stomach tissue lysate, human colon tissue, mouse colon tissue, rat colon tissue, A431.

Molecular wt :

Predicted band size: 131 kDa

Subcellular location :

Secreted, extracellular space, extracellular matrix, basement membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:200 ;FC: 1:5,000 ;IF-Tissue: 1:50 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722815_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMC2 antibody (HA722815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722815_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-LAMC2 antibody (HA722815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722815_6.jpg

Pic legend6 :

Immunocytochemistry analysis of A431 (positive) and A549 (low expression) labeling LAMC2 with Rabbit anti-LAMC2 antibody (HA722815) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAMC2 antibody (HA722815) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722815_7.jpg

Pic legend7 :

Flow cytometric analysis of A549 (left, low expression) and A431 (right, positive) cells labeling LAMC2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722815, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722815_8.jpg

Pic legend8 :

LAMC2 was immunoprecipitated from 0.2 mg A431 cell lysate with HA722815 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722815 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA722815 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA722815 in A431 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801

more info or order :

HUABIO product webpage