Hormone sensitive lipase / HSL | HUABIO HA722810 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Hormone sensitive lipase / HSL

catalog :

HA722810

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-22

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 117 kDa Observed band size: 90 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (negative) Lane 2: Mouse brown adipose tissue lysate Lane 3: Rat brown adipose tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 117 kDa Observed band size: 90 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722810

Target name :

Hormone sensitive lipase / HSL

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human HSL aa 931-980.

Uniprot id :

Q05469>SwissProt: Q05469 Human;SwissProt: P54310 Mouse;SwissProt: P15304 Rat

Host :

Rabbit

Clone number :

PSH07-22

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, SH-SY5Y cell lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, human breast tissue, mouse breast tissue, rat breast tissue.

Molecular wt :

Predicted band size: 117 kDa

Subcellular location :

Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:30,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722810_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722810_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722810_6.jpg

Pic legend6 :

Hormone sensitive lipase / HSL was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722810 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722810 at 1/3,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722810 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722810 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801

more info or order :

HUABIO product webpage