Phospho-Cofilin (S3) | HUABIO HA722806 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Cofilin (S3)

catalog :

HA722806

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-18

reactivity :

human, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Cofilin (S3) on different lysates with Rabbit anti-Phospho-Cofilin (S3) antibody (HA722806) at 1/2,000 dilution. Lane 1: COS-1 cell lysate Lane 2: C6 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: A431 cell lysate Lane 6: A431 cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722806) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722806

Target name :

Phospho-Cofilin (S3)

Species reactivity :

Human,Rat,Monkey

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser3 of human Cofilin aa 1-50.

Uniprot id :

P23528>SwissProt: P23528 Human;SwissProt: P45592 Rat

Host :

Rabbit

Clone number :

PSH07-18

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

COS-1 cell lysate, C6 cell lysate, Jurkat cell lysate, MCF7 cell lysate, A431 cell lysate.

Molecular wt :

Predicted band size: 19 kDa

Subcellular location :

Nucleus matrix, Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, lamellipodium membrane, lamellipodium, growth cone, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage