Phospho-DRP1 (S616) | HUABIO HA722760 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-DRP1 (S616)

catalog :

HA722760

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-77

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-DRP1 (S616) on different lysates with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100ng/mL nocodazole for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 75 kDa Exposure time: Lane 1-4 (left): 1 minute 30 seconds; ECL: K1801; Exposure time: Lane 1-4 (right): 1 minute 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722760) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa cells labeling Phospho-DRP1 (S616). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722760, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Western blot analysis of Phospho-DRP1 (S616) on different lysates with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: PC-12 cell lysate Lane 4: HeLa cell lysate, the membrane blocked with phospho-peptide Lane 5: C2C12 cell lysate, the membrane blocked with phospho-peptide Lane 6: PC-12 cell lysate, the membrane blocked with phospho-peptide Lane 7: HeLa cell lysate, the membrane blocked with non-phospho-peptide Lane 8: C2C12 cell lysate, the membrane blocked with non-phospho-peptide Lane 9: PC-12 cell lysate, the membrane blocked with non-phospho-peptide Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722760) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722760

Target name :

Phospho-DRP1 (S616)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser616 of human DRP1.

Uniprot id :

O00429>SwissProt: O00429 Human;SwissProt: Q8K1M6 Mouse;SwissProt: O35303 Rat

Host :

Rabbit

Clone number :

PSH06-77

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL nocodazole for 18 hours cell lysate, HeLa, C2C12 cell lysate, PC-12 cell lysate, mouse brain tissue, Jurkat cell lysate, HEK-293 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, rat brain tissue, PC-12.

Molecular wt :

Predicted band size: 82 kDa

Subcellular location :

Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:10,000 ;IF-Tissue: 1:500-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722760_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722760) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722760_5.jpg

Pic legend5 :

Western blot analysis of Phospho-DRP1 (S616) on different lysates with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: PC-12 cell lysate Lane 7: Human brain tissue lysate Lane 8: Mouse brain tissue lysate Lane 9: Mouse heart tissue lysate Lane 10: Rat brain tissue lysate Lane 11: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 75 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722760_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722760) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722760_7.jpg

Pic legend7 :

Immunocytochemistry analysis of HeLa cells labeling Phospho-DRP1 (S616) with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722760_8.jpg

Pic legend8 :

Immunocytochemistry analysis of PC-12 cells labeling Phospho-DRP1 (S616) with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-DRP1 (S616) antibody (HA722760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage