Hex | HUABIO HA722753 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Hex

catalog :

HA722753

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE37-45

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Hex on different lysates with Rabbit anti-Hex antibody (HA722753) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: MCF7 cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 32 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Hex was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA722753 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722753 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA722753 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA722753 in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1801

product information

SKU :

HA722753

Target name :

Hex

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Hex aa 91-240 / 270.

Uniprot id :

Q03014>SwissProt: Q03014 Human

Host :

Rabbit

Clone number :

JE37-45

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HT-29 cell lysate.

Molecular wt :

Predicted band size: 30 kDa

Subcellular location :

Nucleus, nuclear body, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage