CD68 | HUABIO HA722737 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD68

catalog :

HA722737

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-65

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 35 kDa Observed band size: 90-200 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

product information

SKU :

HA722737

Target name :

CD68

Species reactivity :

Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse CD68 aa 1-316.

Uniprot id :

P31996>SwissProt: P31996 Mouse;Entrez Gene: 287435 Rat

Host :

Rabbit

Clone number :

PSH06-65

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RAW264.7 cell lysate, M‑NFS‑60 cell lysate, J774A.1 cell lysate, Rat spleen tissue lysate, RAW264.7, mouse brain tissue, mouse intestine tissue, mouse spleen tissue, rat intestine tissue, rat spleen tissue.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Endosome membrane, Lysosome membrane; Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:500 ;IF-Tissue: 1:500 ;IHC-Fr: 1:1,000

Advanced Validation :

Relative expression (RE),Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722737_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722737_5.jpg

Pic legend5 :

Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CD68 with Rabbit anti-CD68 antibody (HA722737) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD68 antibody (HA722737) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722737_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722737_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722737_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA722737_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat intestine tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA722737_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA722737_11.jpg

Pic legend11 :

Application: IF-tissue Species: Mouse Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA722737_12.jpg

Pic legend12 :

Application: IF-tissue Species: Rat Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage