Slow Skeletal Myosin Heavy chain | HUABIO HA722735 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Slow Skeletal Myosin Heavy chain

catalog :

HA722735

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-54

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/1,000 dilution. Lane 1: Rat skeletal muscle tissue lysate Lane 2: Rat spleen tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 223 kDa Observed band size: 223 kDa Exposure time: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722735) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722735

Target name :

Slow Skeletal Myosin Heavy chain

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human MYH7 aa 1,250-1,300.

Uniprot id :

P12883>SwissProt: P12883 Human;SwissProt: Q91Z83 Mouse;SwissProt: P02564 Rat

Host :

Rabbit

Clone number :

PSH07-54

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat skeletal muscle tissue lysate, human heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue, rat heart tissue.

Molecular wt :

Predicted band size: 223 kDa

Subcellular location :

Cytoplasm, myofibril, sarcomere.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:2,000 ;IF-Tissue: 1:500 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722735_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722735_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722735_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722735_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722735) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722735_8.jpg

Pic legend8 :

Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722735, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA722735_9.jpg

Pic legend9 :

Immunofluorescence analysis of frozen mouse skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722735, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA722735_10.jpg

Pic legend10 :

Immunofluorescence analysis of frozen rat skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722735) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722735, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage