product summary
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company name :
HUABIO
product type :
antibody
product name :
PDZK1
catalog :
HA722729
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE37-70
reactivity :
human
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of PDZK1 on different lysates with Rabbit anti-PDZK1 antibody (HA722729) at 1/1,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 70 kDa
Exposure time: Lane 1: 16 seconds; Lane 2: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
PDZK1 was immunoprecipitated from 0.2 mg Caco-2 cell lysate with HA722729 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722729 at 1/500 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Caco-2 cell lysate (input)
Lane 2: HA722729 IP in Caco-2 cell lysate
Lane 3: Rabbit IgG instead of HA722729 in Caco-2 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 44 seconds; ECL: K1801
product information
SKU :
HA722729
Target name :
PDZK1
Species reactivity :
Human
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human PDZK1 aa 470-519 / 519.
Uniprot id :
Q5T2W1>SwissProt: Q5T2W1 Human
Host :
Rabbit
Clone number :
JE37-70
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Caco-2 cell lysate, Human liver tissue lysate.
Molecular wt :
Predicted band size: 57 kDa
Subcellular location :
Membrane, Cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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