ATF6 | HUABIO HA722722 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATF6

catalog :

HA722722

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-59

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATF6 on different lysates with Rabbit anti-ATF6 antibody (HA722722) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: HUVEC cell lysate Lane 4: 293T cell lysate Lane 5: HepG2 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse pancreas tissue lysate Lane 9: Mouse kidney tissue lysate Lane 10: Rat pancreas tissue lysate Lane 11: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 75 kDa Observed band size: 100-110 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722722) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-ATF6 antibody (HA722722) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722722) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ATF6 antibody (HA722722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722722

Target name :

ATF6

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ATF6 aa 1-350.

Uniprot id :

P18850>SwissProt: P18850 Human;Entrez Gene: 226641 Mouse;Entrez Gene: 304962 Rat

Host :

Rabbit

Clone number :

PSH06-59

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A431 cell lysate, HUVEC cell lysate, 293T cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse pancreas tissue lysate, Mouse kidney tissue lysate, Rat pancreas tissue lysate, Rat kidney tissue lysate, rat pancreas tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 75 kDa

Subcellular location :

Endoplasmic reticulum membrane, Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722722_4.jpg

Pic legend4 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 1μM Thapsigargin for 1 hour with ATF6 (HA722722) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage