TNFRSF14/HVEM | HUABIO HA722678B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TNFRSF14/HVEM

catalog :

HA722678B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH06-42

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human HVEM matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722678B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native HVEM in human serum samples. The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum.

image 3 :

Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract.

product information

SKU :

HA722678B

Target name :

TNFRSF14/HVEM

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Human TNFRSF14 aa 39-202.

Uniprot id :

Q92956>SwissProt: Q92956 Human

Host :

Rabbit

Clone number :

PSH06-42

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant standard Human TNFRSF14 protein (HA210556).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-41] to Human TNFRSF14 antibody (Capture) (HA722676) and recombinant standard Human TNFRSF14 protein (HA210556) as the standard. The reference range value is 10.3-2,500 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722678B_4.png

Pic legend4 :

Interpolated concentrations of spiked HVEM in human cell culture media samples. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage