Phospho-p38 alpha (T180 + Y182) | HUABIO HA722669 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-p38 alpha (T180 + Y182)

catalog :

HA722669

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-35

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-p38 alpha (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/2,000 dilution and p38 alpha antibody (ET1702-65) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Jurkat treated with UV for 1 hour cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722669) at 1/2,000 dilution and p38 alpha antibody (ET1702-65) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-p38 alpha (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane) Lane 2: PC-12 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minute 30 secons; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722669) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Jurkat cells treated with UV for 1 hour labeling Phospho-p38 alpha (T180 + Y182) with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA722669

Target name :

Phospho-p38 alpha (T180 + Y182)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 alpha / MAPK14.

Uniprot id :

Q16539>SwissProt: Q16539 Human;SwissProt: P47811 Mouse;SwissProt: P70618 Rat

Host :

Rabbit

Clone number :

PSH06-35

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, Jurkat treated with UV for 1 hour cell lysate, NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Jurkat cells treated with UV for 1 hour, NIH/3T3 cells treated with 25μg/mL Anisomycin for 30 minutes.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:100 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722669_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells treated with 25μg/mL Anisomycin for 30 minutes labeling Phospho-p38 alpha (T180 + Y182) with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA722669) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722669_5.jpg

Pic legend5 :

Flow cytometric analysis of Jurkat cells untreated (left) or treated (right) with UV for 1 hour labeling Phospho-p38 alpha (T180 + Y182). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722669, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage