SFT | HUABIO HA722578 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SFT

catalog :

HA722578

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE64-46

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: Raji cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722578) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

SFT was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722578 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722578 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722578 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722578 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds; ECL: K1801

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SFT antibody (HA722578) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722578

Target name :

SFT

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human SFT aa 98-147 / 147.

Uniprot id :

P51668>SwissProt: P51668 Human;SwissProt: P61080 Mouse;SwissProt: D3ZDK2 Rat

Host :

Rabbit

Clone number :

JE64-46

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, K-562 cell lysate, Raji cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, human thyroid gland carcinoma tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000-1:2,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722578_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage