product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-MCM2 (S27)
catalog :
HA722574
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE63-40
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Phospho-MCM2 (S27) on different lysates with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse thymus tissue lysate (40 µg/Lane)
Lane 5: Rat thymus tissue lysate (40 µg/Lane)
Lane 6: HeLa cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of HeLa cells labeling Phospho-MCM2 (S27) with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722574) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722574
Target name :
Phospho-MCM2 (S27)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser27 of Human MCM2.
Uniprot id :
P49736>SwissProt: P49736 Human;SwissProt: P97310 Mouse;Entrez Gene: 312538 Rat
Host :
Rabbit
Clone number :
JE63-40
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, HeLa, human cervical carcinoma tissue, human lymph node tissue, mouse lymph node tissue, rat lymph node tissue.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:100
;IHC-P: 1:1,000-1:3,000
;IP: 1-2μg/sample
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722574_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722574) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722574_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722574) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA722574_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat lymph node tissue with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722574) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA722574_7.jpg
Pic legend7 :
Phospho-MCM2 (S27) was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722574 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722574 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722574 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722574 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 20 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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