Phospho-Smad1/5/9 (S463/S465/S467) | HUABIO HA722566 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Smad1/5/9 (S463/S465/S467)

catalog :

HA722566

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE59-46

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Smad1/5/9 (S463/S465/S467) on different lysates with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate Lane 3: HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 4: NIH/3T3 cell lysate Lane 5: NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722566) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of Phospho-Smad1/5/9 (S463/S465/S467) on different lysates with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 10ng/mL BMP4 for 1 hour cell lysate Lane 3: C6 treated with 10ng/mL BMP4 for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722566

Target name :

Phospho-Smad1/5/9 (S463/S465/S467)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD1 and SMAD5 protein.

Uniprot id :

Q15797>SwissProt: Q15797 Human;SwissProt: Q99717 Human;SwissProt: O15198 Human;SwissProt: P70340 Mouse;SwissProt: P97454 Mouse;SwissProt: Q9JIW5 Mouse;SwissProt: O54835 Rat;SwissProt: P97588 Rat;SwissProt: Q9R1V3 Rat

Host :

Rabbit

Clone number :

JE59-46

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate, NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate, human testis tissue, C6 treated with 10ng/mL BMP4 for 1 hour cell lysate, HeLa cells treated with 10ng/mL BMP4 for 1 hour, NIH/3T3 cells treated with 10ng/mL BMP4 for 1 hour.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722566_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells treated with 10ng/mL BMP4 for 1 hour labeling Phospho-Smad1/5/9 (S463/S465/S467) with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722566_5.jpg

Pic legend5 :

Immunocytochemistry analysis of NIH/3T3 cells treated with 10ng/mL BMP4 for 1 hour labeling Phospho-Smad1/5/9 (S463/S465/S467) with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody (HA722566) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage