Engrailed 1 | HUABIO HA722546 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Engrailed 1

catalog :

HA722546

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE37-39

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722546) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Engrailed 1 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722546 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722546 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Rat brain tissue lysate (input) Lane 2: HA722546 IP in rat brain tissue lysate Lane 3: Rabbit IgG instead of HA722546 in rat brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 50 seconds; ECL: K1801

product information

SKU :

HA722546

Target name :

Engrailed 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Engrailed 1 aa 343-392 / 392.

Uniprot id :

Q05925>SwissProt: Q05925 Human;SwissProt: P09065 Mouse;Entrez Gene: 685360 Rat

Host :

Rabbit

Clone number :

JE37-39

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage