product summary
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company name :
HUABIO
product type :
antibody
product name :
ITCH / AIP4
catalog :
HA722530
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE31-09
reactivity :
human, mouse, rat
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA722530 image 1
Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: HeLa cell lysate Lane 3: Ramos cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA722530 image 2
Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ITCH / AIP4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 90 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA722530
Target name :
ITCH / AIP4
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human ITCH / AIP4 aa 1-50 / 903.
Uniprot id :
Q96J02>SwissProt: Q96J02 Human;SwissProt: Q8C863 Mouse;Entrez Gene: 311567 Rat
Host :
Rabbit
Clone number :
JE31-09
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SiHa cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate.
Molecular wt :
Predicted band size: 103 kDa
Subcellular location :
Cell membrane, Cytoplasm, Nucleus, Early endosome membrane, Endosome membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!