product summary
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company name :
HUABIO
product type :
antibody
product name :
Somatostatin Receptor 2
catalog :
HA722521
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE03-37
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 2 :
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722521
Target name :
Somatostatin Receptor 2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,IHC-Fr
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Somatostatin Receptor 2 aa 320-369 / 369.
Uniprot id :
P30874>SwissProt: P30874 Human;SwissProt: P30875 Mouse;SwissProt: P30680 Rat
Host :
Rabbit
Clone number :
JE03-37
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, human brain tissue, rat brain tissue.
Molecular wt :
Predicted band size: 41 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200
;IF-Tissue: 1:100
;IHC-Fr: 1:500
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722521_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722521_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA722521_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Somatostatin Receptor 2 with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA722521_7.jpg
Pic legend7 :
Western blot analysis of Somatostatin Receptor 2 on different lysates with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 60-75 kDa
Exposure time: Lane 1-2: 2 minutes; Lane 3-4: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722521) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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