COL1A1 | HUABIO HA722517 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

COL1A1

catalog :

HA722517

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-20

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. Lane 1: HFF-1 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Mouse skin tissue lysate (40 µg/Lane) Lane 5: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 139 kDa Observed band size: 200/139 kDa Exposure time: Lane 1-4: 1 minute; Lane 5: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722517) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA722517) at 1/2,000 dilution. Lane 1: HFF-1 cell lysate (RIPA buffer) Lane 2: HFF-1 cell lysate (Hot lysis buffer) Predicted band size: 139 kDa Observed band size: 200 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722517) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Fluorescence multiplex immunohistochemical analysis of human liver (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31(M1511-8, Red), anti-COL1A1(HA722517, Magenta) and anti-αSMA (ET1607-53, Yellow) on human liver. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of M1511-8 (1/1000 dilution), HA722517 (1/10000 dilution) and ET1607-53 (1/5000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95C. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

product information

SKU :

HA722517

Target name :

COL1A1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IF-Cell,FC,IP,mIHC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human COL1A1 aa 1197-1208.

Uniprot id :

P02452>SwissProt: P02452 Human;SwissProt: P11087 Mouse;SwissProt: P02454 Rat

Host :

Rabbit

Clone number :

PSH06-20

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HFF-1 cell lysate, NIH/3T3 cell lysate, Human lung tissue lysate, Mouse skin tissue lysate, Rat skin tissue lysate, HFF-1, human colon cancer tissue, human kidney tissue, human lung tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue.

Molecular wt :

Predicted band size: 139 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;IF-Cell: 1:500 ;FC: 1:1,000 ;IP: 1-2μg/sample ;mIHC: 1:1,000 ;IHC-Fr: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722517_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HFF-1 cells labeling COL1A1 with Rabbit anti-COL1A1 antibody (HA722517) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL1A1 antibody (HA722517) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722517_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722517_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722517_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722517_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA722517_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA722517_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA722517_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722517) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA722517_12.jpg

Pic legend12 :

Application: IF-tissue Species: Mouse Site: Kidney Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA722517_13.jpg

Pic legend13 :

Flow cytometric analysis of HFF-1 cells labeling COL1A1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722517, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA722517_14.jpg

Pic legend14 :

COL1A1 was immunoprecipitated from 0.2 mg HFF-1 cell lysate with HA722517 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722517 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HFF-1 cell lysate (input) Lane 2: HA722517 IP in HFF-1 cell lysate Lane 3: Rabbit IgG instead of HA722517 in HFF-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1802

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA722517_15.jpg

Pic legend15 :

Application: IHC-Fr Species: Mouse Site: Lung Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

more info or order :

HUABIO product webpage