Sortilin / NT3 | HUABIO HA722513 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Sortilin / NT3

catalog :

HA722513

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-16

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Sortilin / NT3 on different lysates with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: SK-Br-3 cell lysate (20 µg/Lane) Lane 6: U-2 OS cell lysate (20 µg/Lane) Lane 7: U-87 MG cell lysate (20 µg/Lane) Lane 8: Neuro-2a cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (40 µg/Lane) Lane 11: Mouse lung tissue lysate (40 µg/Lane) Lane 12: Rat brain tissue lysate (40 µg/Lane) Lane 13: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722513) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722513

Target name :

Sortilin / NT3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Sortilin aa 78-755 (Extracellular).

Uniprot id :

Q99523>SwissProt: Q99523 Human;SwissProt: Q6PHU5 Mouse;SwissProt: O54861 Rat

Host :

Rabbit

Clone number :

PSH06-16

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SW480 cell lysate, HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, U-2 OS cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse lung tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, human testis tissue, human brain tissue, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 92 kDa

Subcellular location :

Cell membrane. Endoplasmic reticulum. Endosome. Golgi apparatus. Lysosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722513_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722513_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage