Pyrin | HUABIO HA722509 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Pyrin

catalog :

HA722509

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-12

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Pyrin on different lysates with Rabbit anti-Pyrin antibody (HA722509) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722509) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of THP-1 cells labeling Pyrin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722509, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722509

Target name :

Pyrin

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Pyrin aa 450-781.

Uniprot id :

O15553>SwissProt: O15553 Human

Host :

Rabbit

Clone number :

PSH06-12

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, THP-1 treated with 1μg/mL LPS for 24 hours cell lysate, THP-1.

Molecular wt :

Predicted band size: 86 kDa

Subcellular location :

Cell projection. Cytoplasm. Cytoskeleton. Microtubule. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage