Human LIF | HUABIO HA722496 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human LIF

catalog :

HA722496

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-97

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human LIF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722495) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human LIF protein (HA210736) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722496, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native LIF in cell culture supernatant and serum samples. LIF was measured in 100% cell supernatant. The concentrations of LIF were interpolated from the LIF standard curves. The mean LIF concentration was determined to be 148 pg/mL in MDA-MB-231 cell supernanant. There was no detectable signal in T-47D cell supernatant and human serum.

image 3 :

Interpolated concentrations of native LIF in human A375 cell culture supernatant samples. Interpolated concentration of native LIF was measured in duplicate at different sample concentrations. Undiluted samples were 12.5% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2).

product information

SKU :

HA722496

Target name :

Human LIF

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human LIF aa 23-202 .

Uniprot id :

P15018>SwissProt: P15018 Human

Host :

Rabbit

Clone number :

PSH05-97

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant standard Human LIF protein (HA210736).

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-71] to Human LIF (Capture) (HA722423) and recombinant standard Human LIF protein (HA210736) as the standard. The reference range value is 8.2-2,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722496_4.png

Pic legend4 :

Interpolated concentrations of spiked LIF in human cell culture media samples. The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage