product summary
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company name :
HUABIO
product type :
antibody
product name :
ABO
catalog :
HA722455
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE39-25
reactivity :
human, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (HA722455) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ABO was immunoprecipitated from 0.2 mg 293T cell lysate with HA722455 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722455 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (input)
Lane 2: HA722455 IP in 293T cell lysate
Lane 3: Rabbit IgG instead of HA722455 in 293T cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 2 seconds; ECL: K1801
product information
SKU :
HA722455
Target name :
ABO
Species reactivity :
Human,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ABO aa 1-50 / 354.
Uniprot id :
P16442>SwissProt: P16442 Human
Host :
Rabbit
Clone number :
JE39-25
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HT-29 cell lysate, K-562 cell lysate, 293T cell lysate, rat skeletal muscle tissue lysate.
Molecular wt :
Predicted band size: 41 kDa
Subcellular location :
Golgi apparatus, Golgi stack membrane. Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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