product summary
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company name :
HUABIO
product type :
antibody
product name :
HSPA12A
catalog :
HA722425
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE01-78
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of HSPA12A on different lysates with Rabbit anti-HSPA12A antibody (HA722425) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (10 µg/Lane)
Lane 2: U-87 MG cell lysate (10 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 1 minute 34 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722425) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722425
Target name :
HSPA12A
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human HSPA12A aa 101-150 / 675.
Uniprot id :
O4$3301>SwissProt: O4$3301 Human;SwissProt: Q8K0U4 Mouse;Entrez Gene: 307997 Rat
Host :
Rabbit
Clone number :
JE01-78
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 cell lysate, U-87 MG cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Molecular wt :
Predicted band size: 75 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:500
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722425_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722425_5.jpg
Pic legend5 :
HSPA12A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722425 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722425 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722425 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722425 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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