Human IL-2 Receptor alpha | HUABIO HA722386 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human IL-2 Receptor alpha

catalog :

HA722386

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-66

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human IL2RA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722385) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human IL2RA protein (HA210771) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722386, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native IL2RA in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 2 days. The concentrations of IL2RA measured in duplicate and interpolated from the IL2RA standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL2RA concentration was determined to be 1,306.5 pg/ml in PHA-M stimulated PBMC cell culture supernatant and 101.3 pg/ml in the unstimulated PBMC control.

image 3 :

Interpolated concentrations of native IL2RA in human serum samples. The concentrations of IL2RA were measured in triplicates, interpolated from the IL2RA standard curve and corrected for sample dilution. Undiluted samples are human serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL2RA concentration was determined to be 3,193 pg/mL in human serum.

product information

SKU :

HA722386

Target name :

Human IL-2 Receptor alpha

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IL-2 Receptor alpha aa 22-240 (Extracellular).

Uniprot id :

P01589>SwissProt: P01589 Human

Host :

Rabbit

Clone number :

PSH05-66

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant standard Human IL2RA protein (HA210771).

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-65] to Human IL2RA (Capture) (HA722385) and recombinant standard Human IL2RA protein (HA210771). The reference range value is 8.2-2000pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722386_4.png

Pic legend4 :

Interpolated concentrations of spiked IL2RA in human cell culture media samples. The concentrations of IL2RA were measured in triplicates, interpolated from the IL2RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

more info or order :

HUABIO product webpage